The project is organized in 5 tasks. The first one will consist in optimizing cells immobilization methods using a new methodology (paper submitted in Advance material) for their study in AFM. The second task will be dedicated to the biophysics of the cell wall under different stress conditions. The third task aims at functionalizing AFM tips. Task 4 entitled cell wall functional analyses, will consist in making cell surface maps of the 4 different proteins described before. coordination and management will be the last task lead by the coordinator of the project: Dr E Dague from LAAS-CNRS
This research proposal is clearly fundamental, though deserves high interest in biotechnology applications for health (eg antifungal action) and food (yeast cell wall used as binders to mycotoxins) and henceforth, it will be essentially disseminated to the scientific community through publication in high impact Journals.
