AFM and living cells: from immobilization to single molecule force spectroscopy
Découvrez le calendrier des séminaires interne NBS
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25 mars 2010 : Samuel Guillon
Fabrication et caractérisation
de nanosystèmes électromécaniques à actionnement et détection piézoélectrique
intégré
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1er avril 2010 : Thierry
Leïchlé
Diélectrophorèse et nanofentes
pour le filtrage et la concentration de biomolécules
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8 avril 2009 : Amélie Beduer
La domestication des neurones,
entre fouet et carotte
Nanopatterning molecularly imprinted polymers by soft lithography: a hierarchical approach
Lalo H., Ayela C., Dague E., Haupt K.
Programme ANR jeune chercheur : projet AFMYST
Le projet transdisciplinaire AFMYST se positionne à l’interface de la biologie, de la chimie et de la physique. La question biologique posée concerne l’organisation et la morphogénèse de la paroi des levures Saccharomyces cerevisiae. Cette paroi cellulaire a été essentiellement étudiée par des méthodes de biochimie ou de biologie moléculaire. Notre objectif est d’apporter à cette connaissance les observations en microscopie à force atomique donnant accès à la biophysique de la paroi.
Atelier Interuniversitaire de Microéléctronique
Inauguration du centre Pierre Potier : ITAV
Séminaire annuel du groupe NBS
Etape toulousaine du Débat public sur les Nanotechnologies
Du 30 août au 3 septembre : International conference on molecular mechanisms of fungal cell wall biogenesis
Dague E., Ranchon H.,
François J-M., β-glucans, mannans and chitin relative influence on Sacharomyces cerevisiae cell wall
nanomechanical properties (communication affichée)
Nanoscale VII
Using SPM and related techniques
Oral Presentation
2nd ESF/UB European Summer School in Nanomedecine
Quinta da Marinha (Cascais, Lbonne-Portugal)
Communication : E Dague
Nanotechnologies for exploring living cells
Nanowerk spotlights our recent paper in Langmuir entitled Nanomechanical properties of dead or alive single patterned bacteria
| Dead or alive - nanotechnology technique tells the difference | |
| (Nanowerk Spotlight) A major concern in microbiology is to determine whether a bacterium is dead or alive. This crucial question has major consequences in food industry, water supply or health care. While culture-based tests can determine whether bacteria can proliferate and form colonies, these tests are time-consuming and work poorly with certain slow-growing or non-culturable bacteria. They are not suitable for applications where real-time results are needed, e.g. in industrial manufacturing or food processing. | |
| A team of scientists in France has now discovered that living and dead cells can be discriminated with a nanotechnology technique on the basis of their cell wall nanomechanical properties. This finding is totally new and has been made possible thanks to an interdisciplinary approach which mixes physics, biology and chemistry. This work is a key stone in the understanding of bacterial cell wall behavior. |
programme complet des séminaires
LAAS-CNRS club des affiliés, Pole CancerBioSanté
Communication orale : A. Bancaud, E. Dague
Nouvelles méthodes d'imagerie dynamique en 3 dimensions appliquées aux cellules vivantes
Communication Orale, E Dague :
Working on living cells with the Atomic Force Microscope
Langmuir IF = 4.009
Vol. 24 , Issue 23, 13254-13257
Combining Convective/Capillary Deposition and AFM Oxidation Lithography for Close-Packed Directed Assembly of Colloids
L. Ressier, B. Viallet, A. Beduer, D. Fabre, L. Fabie, E. Palleau, and E. Dague
Water Research IF = 3.427
vol.42, 4751-4760
Adhesion of Campylobacter jejuni and Mycobacterium avium onto polyethylene terephtalate (PET) used for bottled waters
Josiane-Aurore Tatchou-Nyamsi-König, Etienne Dague, Martine Mullet, Jérôme F.L. Duval, Fabien Gaboriaud, Jean-Claude Block
Vol. 24, Issue 07
April 1, 2008 Cover
For
more information, see "Combined Use of Atomic Force
Microscopy, X-ray Photoelectron Spectroscopy, and Secondary Ion Mass
Spectrometry for Cell Surface Analysis"
by Etienne Dague, Arnaud Delcorte, Jean-Paul Latgé, and Yves F.
Dufrêne on pages 2955-2959 of this issue. Understanding the
surface properties of microbial
cells is a major challenge of current microbiological research, and a
key to efficiently exploiting them in
biotechnology. The cover shows high-resolution atomic force microscopy
(AFM) images (500 nm × 500 nm)
recorded in aqueous solution for the surface of four different conidia
of the human fungal pathogen Aspergillus
fumigatus, i.e., wild-type conidia, (top left) and three mutants affected in hydrophobin production (other images). By
combining AFM with X-ray photoelectron spectroscopy and secondary ion mass spectrometry, we show that the
native ultrastructure, surface protein and polysaccharide concentrations, and amino acid compositions of the three
mutants are markedly different from those of the wild type, thereby providing novel insight into the cell wall
architecture of A. fumigatus. The results demonstrate the power of using multiple advanced surface analysis
techniques for probing microbial cell surfaces.
Copyright 2008 American Chemical Society
Click here to see the article.
Couverture du Journal, The Analyst (IF=3.198) Mars 2008 (lien article diatomées)
Oral communication : Chemical force microscopy of single living cells ; session: Biological AFM application; chaired by Christian LeGrimmellec
Combined use of atomic force microscopy, X-ray photoelectron spectroscopy and secondary ion mass spectrometry for cell surface analysis
Article accepté dans Langmuir IF=4.009
Simon Scheuring heads a small dynamic research team that is interested in "the structure and assembly of membrane proteins in native membranes studied by atomic force microscopy". This site provides contact address, references of publications, an image gallery, and a download section where you have access to CV and university degree reports.
Discussion sur les enjeux et risques des nanotechnologies suite à la projection du film le silence des nanos de julien Colin :
2 phrases à retenir et sur lesquelles réfléchir :
- si la réponse est les nanotechnologies, alors qu'elle était la question ?
- y avait-il un comité d'éthique pour empecher Cromagnon de fabriquer une massue ?
