Etienne Dague, LAAS-CNRS, Toulouse, France
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October 2017

POST DOC POSITION OPEN

OLIGOMERIZATION STATE OF GPCRs AT THE SURFACE OF LIVING CELLS AS EXPLORED BY ATOMIC FORCE MICROSCOPY

A 33 months post-doctoral position (ANR french funding “GRApHICS”) is available for a highly motivated post-doc fellow to join a research group specialized in the pharmacology of G Protein Coupled Receptor (GPCR) to study the cell surface organization of these receptors in living cells using AFM- single molecule force spectroscopy.

October 2017

Paper accepted in Frontiers in Microbiology

Integration of Biochemical, Biophysical and Transcriptomics Data for Investigating the Structural and Nanomechanical Properties of the Yeast Cell Wall

Marion Schiavone1,2, Sébastien Déjean3, Nathalie Sieczkowski2, Mathieu Castex2, Etienne Dague4 and Jean M. François1*
  • 1Laboratoire d'Ingénierie des Systèmes Biologiques et Procédés, Institut National des Sciences Appliquées de Toulouse, UPS, INP, Université de Toulouse, Toulouse, France
  • 2Lallemand SAS, Blagnac, France
  • 3Institut de Mathématiques de Toulouse, Toulouse, France
  • 4Laboratoire D'analyse et D'architecture des Systèmes du-Centre National de la Recherche Scientifique, Université de Toulouse, Toulouse, France
Heat map representation of association between biophysical-biochemical variables and genes transcripts from the laboratory and industrial strains. Biophysical and biochemical variables are presented on the vertical scale whereas the gene transcripts are on the horizontal scale. Clustering analysis made with the mixOmics tool (http://mixomics.org/) allows grouping biochemical and biophysical variables into 4 groups (horizontal) and into 6 groups with respect to gene transcripts (vertical). Red (blue) indicates high positive (negative) correlation between gene transcript and biochemical/biophysical variables.

Most Cited Micron Articles

The most cited articles published since 2012, extracted from Scopus.

Ranked #7:
Imaging living cells surface and quantifying its properties at high resolution using AFM in QI™ mode
Volume 48, Issue , May 2013, Pages 26-33
L. Chopinet | C. Formosa | M. P. Rols | R. E. Duval | E. Dague

https://www.journals.elsevier.com/micron/most-cited-articles

September 2017

Accepted paper in Brain Structure and Function
Nanoscale structural mapping as a measure of maturation in the murine frontal cortex

G. Smolyakov E. Dague C. Roux M. H. Seguelas C. Gales  J. M. Senard D. N. Arvanitis


Excellent collaboration between I2MC and LAAS-CNRS. Application of AFM technology to explore the brain nanostructure. We used both topographical and mechanical mapping to explore the modification occuring during maturation in the frontal cortex.
Atomic force microscopy (AFM) is emerging as an innovative tool to phenotype the brain. This study
demonstrates the utility of AFM to determine nanomechanical and nanostructural features of the murine dorsolateral frontal cortex from weaning to adulthood. We found an increase in tissue stiffness of the primary somatosensory cortex with age, along with an increased cortical mechanical heterogeneity. To characterize the features potentially responsible for this heterogeneity, we applied AFM scan mode to directly image the topography of thin sections of the primary somatosensory cortical layers II/III, IV and V/VI. Topographical mapping of the cortical layers at successive ages showed progressive smoothing of the surface. Topographical images were also compared with histochemically derived morphological information, which demonstrated the deposition of perineuronal nets, important extracellular components and markers of maturity. Our work demonstrates that high-resolution AFM images can be used to determine the nanostructural properties of cortical maturation, well beyond embryonic and postnatal development. Furthermore, it may offer a new method for brain phenotyping and screening to uncover topographical changes in early stages of  neurodegenerative diseases.

invited talk and Tutorial

at IMRC 2017, Mexico, 19-26 september 2017

tutorial: Atomic Force Microscopy in Biology: Tricks and solutions

Lecture: Biomedical applications of AFM

June 2017

ANR Project granted: GRAPHICs

Probing GPCR architecture on living cells using AFM-single molecule force spectroscopy

Collaborative Project with Céline Gales, I2MC, Emmanuelle Trévisiol, LAAS-CNR and Jean-Marc Azais, Institut de Mathématique de Toulouse

3 years Post-Doc position opens in january 2018

Invited review in

Seminars in Cell and Developmental Biology

Cell biology of microbes and pharmacology of antimicrobial drugs explored by Atomic Force Microscopy

Cécile Formosa-Daguea, b, c, , 1, Raphaël Emmanuel Duvalb, c, d, Etienne Daguea,

a LAAS-CNRS, Université de Toulouse, CNRS, Toulouse, France
b CNRS, UMR 7565, SRSMC, F-54506 Vandœuvre-lès-Nancy, France
c Université de Lorraine, UMR 7565, SRSMC, Faculté de Pharmacie, F-54001 Nancy, France d ABC Platform®, F-54001 Nancy, France

Nanomechanical analysis of the capsule of Klebsiella pneumoniae. (a) Mechanical model for cell indentation of encapsulated bacteria by an AFM tip proposed by Wang et al. In this model, four stages are identified. Capsule polysaccharides are the green lines and the red lines represent fimbriae. Each stage corresponds to a particular regime of the force profile presented on the graph. Stage 1: long-range double layer repulsion between the negatively charged bacterial surface and the negatively charged silicon nitride AFM tip, fitted to double layer theory (green line); Stage 2: steric or electrosteric interaction during compression of cell surface polymers, fitted to the Pincus theory (purple line); Stage 3: elastic deformation of the compacted surface polymers, fitted to the Hertz theory (blue line); and Stage 4: compression of the bacterial cell cytoplasm, fitted to Hooke's law (black line). (b) Indentation curves analysis of Formosa et al. reveals the capsular organization of colistin-resistant K. pneumoniae cells. The inset represent a force curve recorded on a cell in native condition, fitted to the Hertz model. (c) Mechanical model of K. pneumoniae cell indentation proposed by Mularski et al. In this model, first stage consists in long-range double layer interaction between charged tip and surface, then the tip indents the capsular polysaccharide and finally the cytoplasm. The graph represents a typical force profile of K. pneumoniae cell in HEPES buffer (red) with Hooke’s law fit (blue) and derived parameters, bacterial spring constant, Kbacterium = 97 mN/m, and capsule thickness = 340 nm. Reprinted with per- mission from Refs. [35–37].

March 2017


Accepted paper in Journal of Structural Biology


    Biophysical properties of cardiomyocyte surface     explored by multiparametric AFM

Congratulations to Georges for his great work on this topic
                           

This publication is the result of a great collaboration between, LAAS and ITAV-CNRS, I2MC-INSERM.
                                  
Elasticity map of a cardiomyocyte recorded in AFM QNM mode (on the left) and its related analysis by fourrier transformation (on the right) demonstrating the structural periodicity.

January 2017

Campus France, chargé de la gestion des Partenariats Hubert Curien (PHC), pour le compte du ministère des Affaires étrangères et du développement international, a le plaisir de vous faire savoir que le Comité mixte du Programme AURORA a retenu votre projet au titre de l'exercice 2017.

Elucidating interaction capacities of microorganisms using sensitive force probes


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December 2016

Excellent stay at ITN Mexico for Automatip project

We are really doing good job together. Excellent collaboration

November 2016

We were pleased and proud to receive Adrian Martinez, Karen Genesis and Sergio Proa from Institute Polytechnic of Mexico

to collaborate on our Automatip Project. The Mexican-French exchange is funded by ECOS Nord Committee.




2016 november the 24th

Congratulations to Cécile who received the DGA PhD award. The award has been  given during "Forum de l'innovation DGA" by Jean-Yves Le Drian, ministre de la Défense.


Cécile Formosa, thèse LAAS (Laboratoire d'analyse et architecture des systèmes) au CNRS Toulouse, pour ses travaux visant à « comprendre les cibles d’action des molécules antimicrobiennes à l’aide des nanotechnologies ». Il s’agit d’évaluer l’action de nouvelles molécules antimicrobiennes (antibiotiques et
antifongiques) en utilisant des techniques innovantes de microscopie à force atomique (AFM). L’objectif est de lutter contre l’augmentation de la résistance des bactéries aux antibiotiques observée à l’échelle mondiale, une menace pour la santé publique ainsi que celle des militaires en opérations extérieures.

2016 october the 11th

 PhD Defense: Véronique Lachaize. 




Véronique brillantly defended her PhD thesis.

Congratulations


from the left to the right:
Childérick Severac, Philippe Manivet, Véronique Lachaize, Etienne Dague and Sid Labdi. Sofiane El Kirat-Chatel, the last committy member is missing on the picture.

October 2016

Accepted paper in

ACS applied materials and interfaces

Elasticity, adhesion and tether extrusion on breast cancer cells provide a signature of their invasive potential

Georges Smolyakov,a,b Bénédicte Thiebot,c Clément Campillo*,c Sid Labdi,c Childerick Severac,a Juan Pelta,*c Étienne Dague*a,b

aITAV CNRS, Université de Toulouse, CNRS, UPS, France b LAAS-CNRS, Université de Toulouse, CNRS, Toulouse, France

cLaboratoire d’Analyse et Modélisation pour la Biologie et l'Environnement LAMBE-CNRS, Université d’Evry,

Evry, Université de Cergy-Pontoise, Cergy-Pontoise, France

This publication is the result of a great collaboration between, LAAS and ITAV-CNRS, University of Evry and University of Cergy. Let the show go on and continue this exciting work.

September 2016

Book Chapter in

Handbook of Electroporation,

edited by Damijan Miklavčič

Atomic Force Microscopy to explore Electroporation effects on cells


In this chapter, we review the work of Louise Chopinet, who graduated in 2013, and Flavien Pillet who is currently on a post doc position at IPBS. Many thanks for their contributions, this chapter is dedicated to their work.

http://link.springer.com/referenceworkentry/10.1007/978-3-319-26779-1_134-1

July 2016

New name for our team at LAAS-CNRS:

ELIA Engineering for Life Science Application

June 2016

Organization of Nano In BIO

NanoInBio Advances for life and materials science (LeGosier, Guadeloupe, France, 31 mai – 5 juin 2106)

- Hélène Martin-Yken H., Formosa-Dague C., Schiavone M., François J-M., Dague E., Stress, Drug Resistance and Adhesion: a closer look into the dark side of the wall                                                                                    

- Smolyakov G., Campillo C., Labdi S., Thiebot B., Severac C., Pelta J., Dague E. Cell adhesion and rigidity probed by single-cell force spectroscopy reveal the invasive character of breast cancer lines

- Schiavone M., François J-M., Sletmoen M., Dague E., Interactions between concanavalin A and yeast cell wall mutants studied by Optical Tweezers and Atomic Force microscopy                                  

- Pillet F., Formosa-Dague C., Teissié J., Rols M-P., Dague E. AFM analysis of cell-wall damages induced on bacteria by Pulsed Electric Field 

May 2016

Invited speaker at Imaging the Yeast Symposium

Dague E., Imaging living yeast cells and quantifying their biophysical properties by atomic force microscopy


                         

April 2016

Opened PhD position in the team

NANOCARD II

Caractérisation de la membrane latérale du cardiomyocyte à l’échelle nanométrique en physio-physiopathologie cardiaque

March 2016: France-Mexico Cooperation


ECOS Nord Project awarded to Etienne, Childérick and Adrian

Nano-palpation pour le diagnostic

- France : L'Institut des Technologies Avancées en sciences du Vivant (ITAV) du Centre National de la Recherche scientifique (CNRS)

- Mexique :  Centre de recherche en informatique (CIC) MICROSE lab de l’Institut Polytechnique National (Mexico)


February 2016: IDEX Project awarded to Hélene, Mickael, Céline, Marit and Etienne

SMiRCH:

Force Spectroscopy and Optical Tweezers combined for Single Molecule Research at the Surface of Living Cells

January 2016: coopération France-Norvège

Candidature Åsgard Acceptée


Mon séjour en Norvège est prévu pour octobre 2016!

 Le programme Åsgard est un programme d’invitation de chercheurs français en Norvège géré par la Mission scientifique de l’Institut français.

Le programme Åsgard a pour objectifs de:

  • Développer les échanges scientifiques entre la France et la Norvège
  • Favoriser l’émergence de coopérations en recherche et en transfert de technologie
  • Permettre aux chercheurs français et norvégiens d’étendre leurs réseaux internationaux

November 2015

- Congratulation to Laure and Flavien for their poster award received during 11ème journée du Canceropole Grand Sud Ouest

And

- Invited keynote lecture at NanoBioMed in Spain

11ème journée du cancéropole Grand Sud Ouest (Bordeaux, France, 5-6 novembre 2015)

Pillet F., Gibot L., Madi M., Rols P-P., Dague E., Atomic Force Microscopy: a tool to study native dermis and 3D cellular models
. NanoBioMed 2015 (Barcelone, Spain, 18-20 nov 2015) Dague E. Biomedical Applications of Atomic Force Microscopy            

juillet 2015

Congratulation to Cécile for her PhD award given by "Société Française des microscopistes"

XIVème colloque de la Société Française des Microscopies (Nice, France 30 juin-3 juillet 2015)

Formosa C., Dague E., Comprehension of the mechanisms of actions of antimicrobial drugs using bionantotechnologies (invited plenary lecture)
April 2015

March 2015

Chapter published in the book Advanced Microscopy in mycology
. Dague E., Formosa C., 2015, Imaging living yeasts cells and quantifying their biophysical properties by Atomic Force Microscopy in Springer Eds, Tanya Dahms and Kirk Czymmek

February 2015

3 papers published in

- FEMS Yeast Reserach: congratulations to Marion

- Journal of Molecular Recognition: congratulations to Cécile and Véronqiue

- Nanomedicine NBM: congratulations to Cécile
Schiavone M., Sieczkowski N., Castex M., Dague E., François J-M. 2015 Effects of the strain background and autolysis process on the composition and biophysical properties of the cell wall from two different industrial yeasts. FEMS Yeast Reserach 15,                                     

Formosa C., Lachaize V., Rols M.P., Martin Yken H., François J.M., Duval R.E., Dague E., 2015. Mapping HA-tagged protein at the surface of living cells by atomic force microscopy.
Journal of Molecular Recognition 28. 1-9                                                                     

Formosa C., Schiavone M., Boisramé A., Lavie Richard M., Duval R.E., Dague E., 2015. Multiparametric imaging of adhesive nanodomains at the surface of C. albicans by Atomic Force Microscopy
Nanomedicine NBM                                                                                                   

january 2015

New paper published in Nature Protocols
Formosa C., Pillet F., Schiavone M., Duval R.E., Ressier L., Dague E., 2015.
Generation of living cell arrays for Atomic Force Microscopy studies
Nature Protocols 10. 1. 199-204 


December 2014 :

- Marion Schiavone PhD Thesis

Combination of biochemical, molecular and biophysical approaches to investigate the impact of strain background and production process on the yeast cell wall composition and molecular architecture


- new publication in ACS Applied materials and interfaces

- Oral and Poster presentation at AFMBioMed, SanDiego California USA
Congratulation to Marion Schiavone for the defense of her PhD thesis. She will be hired on a Post Doc position soon by Lallemand SA.


Jauvert E., Palleau E., Dague E., Ressier L.,
Directed assembly of living Pseudomonas aeruginosa on PEI patterns fabricated by nanoxerography for statistical AFM bio-experiments
ACS Applied materials and interfaces 


- Dague E., Three biomedical applications of atomic force microscopy, (Communication orale).

- Formosa C., Duval R.E., Dague E., Unravelling of a mechanism of resistance to colistin in Klebsiella pneumoniae thanks to Atomic Force Microscopy, (Communication orale).

- Lachaize V ;, Genet G., Guilbeau-Frugier C., Severac C., Senard J-M., Gales C., Dague E. AFM-based sarcolemmal surface analysis of living cardiomyocytes unveils unexpected mitochondrial shift in heart failure. (Communication affichée)

September 2014, Cecile Formosa PhD thesis

Comprehension of the mechanisms of action of antimicrobial molecules using nanobiotechnologies


congratulation to Cécile Formosa for the defense of her PhD Thesis
Cécile will stay with us, on a Post-Doc position, for 2 more months.
In Januray she will move on a Post Doc position in the Dufrêne's lab.

July 2014

new Papers accepted in

FEMS YEAST: 

A combined chemical and enzymatic method to determine quantitatively the polysaccharaide components in the cell wall of yeasts

by Schiavone M., Formosa C., Martin Yken H., Dague E., François J-M.

Journal of Molecular Recognition 

Mapping HA-tagged protein at the surface of living cells by atomic force microscopy.

by Formosa C., Lachaize V., Rols M.P., Martin Yken H., François J.M., Duval R.E., Dague E

and Nanomedicine NBM

Multiparametric imaging of adhesive nanodomains at the surface of C. albicans by Atomic Force Microscopy

by Formosa C., Schiavone M., Boisramé A., Lavie Richard M., Duval R.E., Dague E.

June 2014

Welcome to Georgiy Smolyakov. He joined the group at ITAv and is funded by a grant from "Fondation pour la Recherche Médicale" to work on the Nanocardiology project.

This research is supported by FRM, Grant # ING 21040129094


MAY 2014

New paper published in Journal of Molecular and Cellular Cardiology

AFM of living cardiomyocytes surface unveiled unexpected mitochondrial shifts in heart failure

by

Dague E., Genet G., Fauconnier J., Guilbeau-Frugier C., Payré B., Chopinet L., Alsteens D., Severac C., Thireau J., Heymes C., Honton B., Lacampagne A.,Pathak A., Sénard J.M., Galés C.

Forum des microscopistes à sonde locale

17-21 mars 2014 Montauban

February 2014

Oral and poster presentations at the linz winterworkshop

    

Position Available for 2 years

Engineer with excellent skills in AFM applied to living cells

Funded by FRM (Fondation pour la Recherche Médicale), the project aims at exploring the cardiomyocytes organization at the nanoscale using atomic force microscopy. For more details contact

edague@laas.fr and celine.gales@inserm.fr

January 2014 Paper accepted in BMC Biology

Uncovering by AFM an original circular structure at the yeast cell surface in response to heat shock

by F. Pillet, S. Lemonier, M. Schiavone, C. Formosa, H. Martin-Yken, J-M François, E Dague

 The results published in this article have been collected during more than 3 years. We are particularly proud of achieving this nice work. Thank you all for your hard work, patience and tenacity

January 2014

HAPPY NEW YEAR

December 2013

New paper accepted in BBA General Subject

21 novembre 2013

Poster presentation at Forum de l'Innovation of DGA (Direction Générale de l'Armement).

Bionanotechnologies : Comprendre les cibles d'action de nouvelles molécules antibactériennes

Thèse de Cécile Formosa

La direction générale de l’armement (DGA) a organisé son second forum innovation le 21 novembre 2013 sur le campus de l’école polytechnique à Palaiseau. Articulée autour d’un village exposition, d’une séance plénière et d’ateliers thématiques, cette manifestation est l'événement annuel majeur de la recherche et de l’innovation duale.

   

Novembre 2013

Invited lecture in Tronheim, Norway
Intensive course for PhD students from all over Norway within the fields Biophysics, Biotechnolody, Pharmacy, Medicine or related fields. They will gather in Trondheim on these two weeks. Week one they learn about light microscopy based techniques, and week two, which is the week starting with the 11th of November, they learn about force based techniques, involving AFM and optical tweezers.
Dague E., From single cell to single molecule, AFM in life sciences

Octobre 2013

Bienvenue à Véronique Lachaize

Doctorante co encadrée avec Céline Gales de l'I2MC,

membre de l'équipe projet NanoCardiologie à l'ITAV USR 3505

 Véronique effectuera sa thèse sur la thématique de nanocardiologie et dans le cadre de l'équipe projet hebrgée à l'ITAV. Ce recrutement concrétise ma collaboration, à l'ITAV (USR 3505) avec Céline Gales (I2MC) et va permettre de faire avancer notre équipe projet.

October 2013

New publication in Current genetics

September 2013

New publication in PlosOne

24 septembre 2013

Soutenance Thèse Louise Chopinet

Directeurs de Thèse
Marie Pierre Rols, Etienne Dague

Rapporteurs :
Luis Mir, Victor Shahin

Examinateurs :
Bernard Ducommun, Philippe Pouliguen, René Vézinet


ICAAC 2013 in Denver

Etienne was honored as George McCracken Infectious Disease Fellow and received a grant to cover travel expenses
Dague E., Formosa C., Grare M., Duval R.E., Atomic Force Microscopy for microbes investigation and antimicrobial agent evaluation

ICAAC 54th Interscience conference on antimicrobial agents and chemotherapy (Denver, USA, 10-13 septembre 2013




5 juillet 2013

Projet Région Bacteau retenu pour financement par la région Midi Pyrénée

 Ce projet initié et conduit par le C. Causserand du LGC a pour but de mieux comprendre les phénomènes de fuite microbinenne à travers les pores des filtres.
Le travail sera réalisé par Arthur Gaveau, doctorant, et pour ce qui me concerne sera hébergé sur la plate forme de BioNanoTechnologie de l'ITAV USR 3505

Poster communication at PYFF5

5th Conference on Physiology of Yeast and Filamentous Fungi

June 4 - 7, 2013, Montpellier, FRANCE

The yeast response to external stress investigated by Atomic Force Microscopy

C. Formosa, M. Schiavone, C. Elsztein, H. Martin-Yken, Jr M. A. de Morais, R. E. Duval, J. M. François and E. Dague


and

A simple method to determine Saccharomyces cerevisiae cell wall composition by combined chemical and enzymatic hydrolysis

M. Schiavone, J.-M. François

June 2013

New publication accepted in

Biophysica and Biochimica Acta; Biomembranes

Destabilization induced by electropermeabilization analyzed by Atomic Force Microscopy

Louise Chopinet, Charles Roduit , Marie-Pierre Rols and Etienne Dague

Highlights

We demonstrate that AFM can be used to study electroporation.

Electric fields provoke a decrease in membrane stiffness.

Electro-destabilization process measured by AFM happens later than membrane permeabilization

Electro-destabilization last longer than PI uptake observed by fluorescence imaging.

AFM imaging shows the disturbed plasma membrane.

May 23rd 2013


AFM Workshop at Institut du Cerveau et de la Moelle épinière (Paris)

Organized for INSERM by Etienne Dague and Grégory Francius.


 Program

Cellular and molecular nanomechanics by Atomic Force

Microscopy

23 mai 2013 - May 23rd, 2013

Organizers: Etienne Dague (LAAS-CNRS UPR 8001, Toulouse,
France) et Grégory Francius (LCPME UMR 7564, Nancy, France)

May 16th 2013

 L'AFM dans tous ses états

Rencontre de la fédération de recherche FERMaT

April 2013

New publication in Antimicrobial Agent and Chemotherapy

Nanoscale effects of Caspofungin against two yeast species; Saccharomyces cerevisiae and Candida albicans

C. Formosa, M. Schiavone, H. Martin-Yken, J. M. François, R. E. Duval and E. Dague

LAAS-CNRS, LISBP, SRSMC, Plateforme ABS

In this paper, the authors charaterize the effect of caspofungin against S. cerevisiae  and C. albicans. The use of AFM for investigating the effect of antifungal drug is relevant in nanomedicine, as it should help understanding their mechanism of action on the fungal cells, as well as unraveling unexpected effects on cell division and fungal adhesion.



7 février 2013

Publication acceptée dans MICRON

Imaging living cells surface and quantifying its properties at high resolution using AFM in QITM mode

Chopinet, L. and Formosa, C. Rols, M. P. Duval, R. E.5 Dague, E.

This paper presents results obtained using the quantitative imaging mode. We explored the surface topography, nanomechanical or adhesion properties of several single cells from bacteria to eukaryotic cells :
- Escherichia coli
- Candida albicans
- Aspergillus fumigatus
- CHO cells
- cell nuclei
 
All together the results demonstrate that AFM can be conducted on soft and fragile sample, very close to their native environment. All the experiments were conducted in liquid, on living cells, at regulated temperature and pH when required.


Soutenance de Thèse : ERIC JAUVERT

SALLE DES THÈSES DE L'INSA

21 DÉCEMBRE À 10H

Thèse co diriger par Laurence Ressier (LPCNO) et Etienne Dague (LAAS-CNRS)

Sujet : Assemblage dirigé de micro organismes et fonctionnalisation de pointes pour biocaractérisation par AFM

November  2012

Habilitation à Diriger les Recherches

Etienne DAGUE

Docteur de l’université Henri Poincaré Nancy 1, Docteur en Pharmacie,

Chargé de Recherche du CNRS au LAAS-CNRS (UPR8001)

Sujet

Les technologies de Microscopie à Force Atomique appliquées sur les systèmes vivants

         Membres du jury

Rapporteurs :

Dr. Sandor Kasas, EPFL-Univeristé de Lausanne (CH)

Dr. Frank Lafont, DR CNRS INSERM U1019 - CNRS UMR 8204

Dr. Pierre-Emmanuel Milhiet, DR CNRS UMR 5048

Examinateurs

Dr. Jean Pierre Aimé, DR CNRS Université de Bordeaux 1, UMR 5248

Dr. Christian Bergaud, DR CRNS, LAAS-CNRS UPR 8001

Pr. Pierre-Emmanuel Gleizes, Université de Toulouse, LBME UMR 5099

Pr. Christophe Vieu, INSA Université de Toulouse, LAAS-CNRS UPR 8001

September 2012

Academic Editor of PlosOne

September 2012 :

NANOCARDIOLOGY AND BIOPHYSIC OF CANCEROUS CELLS

NEW TEAM IN CENTRE PIERRE POTIER ITAV


August 2012

New paper in Scientific Reports,

Nature Publishing Group

 In this paper by C. Fromosa et al., we studied the effect of antibiotics and CX1 on a Pseudomonas aeruginosa multi-drug resistant strain. We used AFM to study surface topography and roughness of the bacteria submitted to the 3 molecules. To go further, we explored the bacterial cell wall nanoscale organization using functionalized AFM Tips. Finally we have elucidated the mechanism of action of CX1: it destroys the outer membrane of the bacteria as demonstrated by the results on artificial phospholipidic bilayers.

Nanomedecine NBM Impact Factor 2012

IF = 6.692!!

april 2012

New paper in Sensors and Actuators



Mai 2012

New publication in Biofouling


January 2012
New Publication in Circulation Research

Genet G., Guilbeau-Frugier C., Honton B., Dague E., Schneider M.D., Coatrieux C., Calise D., Cardin C., Nieto C., Payré B., Dubroca C., Marck P., Heymes C., Dubrac A., Avranitis C., Despas F., Altié M-F.; Seguelas M-H., Delisle M-B., Davy A., Senard J-M., Pathak A. 

Ephrin B1 is a novel component of the lateral membrane of the cardiomyocyte and is essential for the stability of cardiac architecture cohesion 
Nov 2011
New publication in Biophysical journal

Doan Thanh Lam Le, Yann Guérardel, Pascal Loubière, Muriel Mercier-Bonin and Etienne Dague

Measuring Kinetic Dissociation/Association Constants Between Lactococcus lactis Bacteria and Mucins Using Living Cell Probes
14 oct 2011 Soutenance thèse Lam Doan Thanh LE
à l'INSA département de mécanique S 112
13h30		 Thèse d'université : Diplome de doctorat de l'Université de Toulouse
Identification des déterminants physico-chimiques et biologiques mis en jeu dans l'adhésion de Lactococcus lactis à la mucine, modèle PGM
5-6 octobre 2011

10th International Symposium on Scanning probe microscopy and optical tweezers in life sciences

Berlin
invited talk :
AFM applications in nanomedecine, from microbiology to cardiology

From the left to the right (click to enlarge): Paolo Samori (University of Strasbourg), Torsten Jähnke (JPK), Roderick Lim (Basel University), Małgorzata Lekka (Polish Academy of Sciences), Giovanni Longo (EPFL), Nic Mullin (University of Sheffield), Marit Sletmoen (NTNU Trondheim), Etienne Dague (LAAS-CNRS), Bart Hoogenboom (University College London) and Wenke Zhang (Jilin University).
25 sept 2011
Paper accepted in Nanomedecine NBM

Nanoscale effects of antibiotics
 on P. aeruginosa


 This paper by
C. Formosa, M. Grare, R.E. Duval and E. Dague
From

LAAS, ITAV CNRS, Université de Toulouse
SRSMC, UMR CNRS Nancy Université
CHU Purpan
Describes the nanoscale effects of two majaor antibiotics, commonly used in therapeutic, on a dreadfull bacteria responsible of nosocomial infections : Pseudomonas aeruginosa.
17-20 sept 2011
ICCAC Chicago
51st interscience conference on antimicrobial agents and chemotherapy
Communication affichée
23-27 aout 2011
AFMBIOMED Conference
Oral Communication
AFM applications in nanomedecine, from microbiology to cardiology
3 sept 2011
Paper accepted in Nanotechnology

Assembly of live micro-organisms on microstructured PDMS stamps by convective/capillary deposition for AFM bio-experiments

This paper by
E. Dague, E. Jauvert, L. Laplatine, B. Viallet,
C. Thibault and L. Ressier
From
LAAS, ITAV, CNRS, Université de Toulouse
LPCNO, INSA, Université de Toulouse

describes an original immobilization method for living yeast and Aspergillus conidia. A microstructured PDMS stamp is functionalized or activated by respectively ConA or oxygen plasma. A cell suspension is then dragged over the stamp and the stamp wells are filled with living cells.
mai 2011
ANR Jeune Chercheur AFMYST
retenu pour financement
17 février 2011
Réunion des AFMistes Toulousains
Childérick Severac, ITAV 
Etienne Dague, LAAS-CNRS

17 dec 2010
Séminaire Institut de Biologie Structurale (IBS) Grenoble
18 nov 2010
Cours M2 P : Diagnostic microbiologique : approches innovantes
 BioNanotechnologie
26 oct 2010 Séminaire Juan PELTA
LAMBE, Université d'Evry et Cergy Pontoise
11-14 oct 2010 HOPE IN RED

Host-Pathogen interactions in respiratory Infectious Diseases
Organized in Bangalore INDIA by CEFIPRA :
Indo-French Centre for the promotion of Advanced Research
 Invited talk : Afm Technology for living cells exploration
7-8 oct 2010

Research Networking Programme on

The Functional genomics in Aspergillus fumigatus and new strategies to fight against the first fungal pathogen in Europe
(Fuminomics)

Third Annual Meeting Cell Biology and Biochemistry
Institut Pasteur, Paris, France

October 7-8, 2010
July 2010
Project funded by CNRS interdisciplinar program
THERMOBIOAFM
 This project conducted by E Dague, C. Bergaud and J. Pelta aims at measuring proteins unfolding using AFM at various temperatures. The temperature variation is achived using a thermomicrowire.
Just do it!
Juin 2010
Paper accepted in Langmuir :

Probing in vitro interactions between
Lactococcus lactis and mucins using AFM

 

This paper by

 Etienne Dague Doan Thanh Lam Le Sandrine Zanna, Philippe Marcus, Pascal Loubière, Muriel Mercier-Bonin

 From

 - CNRS ; LAAS Université de Toulouse ;
- LISBP - INRA: UMR792 CNRS: UMR5504
- Laboratoire de Physico-Chimie des Surfaces, CNRS/ENSCP, UMR7045, ce

 describes the fabrication of a cell probe, used to measure the interactions of Lactococcus lactis with mucin, the main glycoprotein of the intestinal mucus.
Project funded by C'Nano GSO

The project “Comparing aptamers and anti-body for single molecule force spectroscopy experiments” will be funded by the network C’Nano Grand Sud Ouest. This project, conducted by E. Dague (LAAS-CNRS), is the result of a budding collaboration with Emmanuelle Trevisiol from LISPB and Jean-Jacques Toulmé from IECB. The project aims at developing the aptamère technology in order to perform single molecule force spectroscopy experiments.

Just do it !
15 mai 2010
Paper accepted in YEAST
An Atomic Force Microscopy analysis of yeast mutants defective in cell wall architecture

This paper by

Etienne Dague, Rajaa Bittar, Hubert Ranchon, Fabien Durand, Hélène Martin Yken
and Jean M François

From

LAAS CNRS, and LISBP, INRA-CNRS-INSA

 is dedicated to the study of the yeast cell wall organization by Atomic Force Microscopy.

Enjoy the reading
12-15 mai 2010  Oral contribution accpeted :
AFM and living cells: from immobilization to single molecule force spectroscopy
Mars 2010 :
Découvrez le calendrier des séminaires interne NBS

- 25 mars 2010 : Samuel Guillon

Fabrication et caractérisation de nanosystèmes électromécaniques à actionnement et détection piézoélectrique intégré

 

- 1er  avril 2010 : Thierry Leïchlé

Diélectrophorèse et nanofentes pour le filtrage et la concentration de biomolécules

 

- 8 avril 2009 : Amélie Beduer

La domestication des neurones, entre fouet et carotte
Février 2010 :  Lab On A Chip IF = 6.478

Nanopatterning molecularly imprinted polymers by soft lithography: a hierarchical approach

Lalo H., Ayela C., Dague E., Haupt K.
Janvier 2010 : Dépot projet AFMYST ANR Jeune Chercheur

Programme ANR jeune chercheur : projet AFMYST

Le projet transdisciplinaire AFMYST se positionne à l’interface de la biologie, de la chimie et de la physique. La question biologique posée concerne l’organisation et la morphogénèse de la paroi des levures Saccharomyces cerevisiae. Cette paroi cellulaire a été essentiellement étudiée par des méthodes de biochimie ou de biologie moléculaire. Notre objectif est d’apporter à cette connaissance les observations en microscopie à force atomique donnant accès à la biophysique de la paroi.
janvier 2010 : 1ère culture cellulaire au Centre Pierre Potier ITAV-Recherche
2-3 dec. 2009
 1er TP Bio-AFM :
Atelier Interuniversitaire de Microéléctronique
18 nov. 2009 :
Inauguration du centre Pierre Potier : ITAV
9 nov. 2009 :
Séminaire annuel du groupe NBS
20 oct. 2009 : Etape toulousaine du Débat public sur les Nanotechnologies
août-sept 2009

 Du 30 août au 3 septembre : International conference on molecular mechanisms of fungal cell wall biogenesis

Dague E., Ranchon H., François J-M., β-glucans, mannans and chitin relative influence on Sacharomyces cerevisiae cell wall nanomechanical properties (communication affichée)
Juillet 2009
Nanoscale VII  
 du 28 au 31 juillet 2009 , Exploring the Future of Nanotechnology
Using SPM and related techniques
Oral Presentation
juin 2009 12-16 juin 2009
2nd ESF/UB European Summer School in Nanomedecine
Quinta da Marinha (Cascais, Lbonne-Portugal)
Communication : E Dague
Nanotechnologies for exploring living cells
26 mai 2009
Nanowerk spotlights our recent paper in Langmuir entitled Nanomechanical properties of dead or alive single patterned bacteria
Dead or alive - nanotechnology technique tells the difference
(Nanowerk Spotlight) A major concern in microbiology is to determine whether a bacterium is dead or alive. This crucial question has major consequences in food industry, water supply or health care. While culture-based tests can determine whether bacteria can proliferate and form colonies, these tests are time-consuming and work poorly with certain slow-growing or non-culturable bacteria. They are not suitable for applications where real-time results are needed, e.g. in industrial manufacturing or food processing.
A team of scientists in France has now discovered that living and dead cells can be discriminated with a nanotechnology technique on the basis of their cell wall nanomechanical properties. This finding is totally new and has been made possible thanks to an interdisciplinary approach which mixes physics, biology and chemistry. This work is a key stone in the understanding of bacterial cell wall behavior.
24 mars 2009 Séminaire Groupe NBS : 1er de la période mars-juin : Explorer une cellule à l'aide des nanotechnologies
programme complet des séminaires
mars 2009
 Cerf A., Cau J.C., Vieu C., Dague E.  Nanomechanical properties of dead or alive single patterned bacteria, accepté le 4 mars dans Langmuir IF 4.001
3 mars 2009 Journée "Des sciences et Technologies de Systèmes vers la Santé"
LAAS-CNRS club des affiliés, Pole CancerBioSanté
Communication orale : A. Bancaud, E. Dague
Nouvelles méthodes d'imagerie dynamique en 3 dimensions appliquées aux cellules vivantes
Janvier 2009,
Prix de l'Académie Nationale de Pharmacie,
13-14 nov 2008 Réunion Annuelle des utilisateurs d'AFM VEECO, 13-14 novembre 2008, Rennes.
Communication Orale, E Dague :
Working on living cells with the Atomic Force Microscope
novembre 2008
 Langmuir IF = 4.009
Vol. 24 , Issue 23, 13254-13257

 
Combining Convective/Capillary Deposition and AFM Oxidation Lithography for Close-Packed Directed Assembly of Colloids
L. Ressier, B. Viallet, A. Beduer, D. Fabre, L. Fabie, E. Palleau, and E. Dague
Septembre 2008
Water Research IF = 3.427
vol.42, 4751-4760
Adhesion of Campylobacter jejuni and Mycobacterium avium onto polyethylene terephtalate (PET) used for bottled waters
Josiane-Aurore Tatchou-Nyamsi-König, Etienne Dague, Martine Mullet, Jérôme F.L. Duval, Fabien Gaboriaud, Jean-Claude Block
Avril 2008

Vol. 24, Issue 07
April 1, 2008 Cover

For more information, see "Combined Use of Atomic Force Microscopy, X-ray Photoelectron Spectroscopy, and Secondary Ion Mass Spectrometry for Cell Surface Analysis" by Etienne Dague, Arnaud Delcorte, Jean-Paul Latgé, and Yves F. Dufrêne on pages 2955-2959 of this issue. Understanding the surface properties of microbial cells is a major challenge of current microbiological research, and a key to efficiently exploiting them in biotechnology. The cover shows high-resolution atomic force microscopy (AFM) images (500 nm × 500 nm) recorded in aqueous solution for the surface of four different conidia of the human fungal pathogen Aspergillus fumigatus, i.e., wild-type conidia, (top left) and three mutants affected in hydrophobin production (other images). By combining AFM with X-ray photoelectron spectroscopy and secondary ion mass spectrometry, we show that the native ultrastructure, surface protein and polysaccharide concentrations, and amino acid compositions of the three mutants are markedly different from those of the wild type, thereby providing novel insight into the cell wall architecture of A. fumigatus. The results demonstrate the power of using multiple advanced surface analysis techniques for probing microbial cell surfaces. 
Copyright 2008 American Chemical Society

Click here to see the article.
mars 2008
Couverture du Journal, The Analyst (IF=3.198) Mars 2008 (lien article diatomées) 
12-15 Février 2008

Oral communication : Chemical force microscopy of single living cells ; session: Biological AFM application; chaired by Christian LeGrimmellec
2 février 2008 Remise du Prix de Thèse de l'Académie des Sciences de Lorraine.
16 janvier 2008

Combined use of atomic force microscopy, X-ray photoelectron spectroscopy and secondary ion mass spectrometry for cell surface analysis

Article accepté dans Langmuir IF=4.009
6 décembre 2007
 Conférence de Simon Scheuring, 16-17h salle de conférence du LAAS.
Simon Scheuring heads a small dynamic research team that is interested in "the structure and assembly of membrane proteins in native membranes studied by atomic force microscopy". This site provides contact address, references of publications, an image gallery, and a download section where you have access to CV and university degree reports.
3 décembre 2007
Le silence des nanos
Discussion sur les enjeux et risques des nanotechnologies suite à la projection du film le silence des nanos de julien Colin :
2 phrases à retenir et sur lesquelles réfléchir :
- si la réponse est les nanotechnologies, alors qu'elle était la question ?
- y avait-il un comité d'éthique pour empecher Cromagnon de fabriquer une massue ?
28 nov 2007
 Article Diatomées accepté dans Environnemental Microbiology IF=4.630